Macvector aligning rnaseq reads5/4/2023 ![]() PPR was first reported in the Ivory Coast in 1942 and is now widespread across sub-Saharan Africa, Arabian Peninsula, Middle East and the Indian sub-continent. Morbidity and mortality in this highly contagious disease is as high as 100% and 90%, respectively. The PPRV genome of 15948 bp encodes six structural proteins, nucleocapsid (N), phosphoprotein (P), matrix (M), fusion (F), hemagglutinin (H) and large (L) protein in 3′ to 5′ direction (3′-N-P-M-F-H-L-5′) and two non-structural proteins (C/V) due to RNA editing of the phosphoprotein gene. PPRV, a linear single stranded, negative sense RNA virus, belongs to the genus Morbilivirus, family Paramyxoviridae. ![]() Peste des petits ruminants (PPR), an acute viral disease of goats and sheep, characterized by fever, erosive stomatitis, conjunctivitis, gastroenteritis and pneumonia is caused by Peste des petits ruminants virus (PPRV). Our findings suggested dysregulation of immune regulatory pathways and genes encoding Transcription Factors (TFs) that govern these pathways in response to viral infection. These data provide insights into the host - PPRV vaccine virus interactome for the first time. Several genes encoding TFs that govern immune regulatory pathways were identified to co-regulate the differentially expressed genes. Network analysis revealed that the protein - protein interaction network among differentially expressed genes is significantly disrupted in infected state. Key genes involved in immune system regulation, spliceosomal and apoptotic pathways were identified to be dysregulated. Functional analysis showed enrichment of immune regulatory pathways under PPRV infection. Comparison of infected transcriptome with control transcriptome revealed 985 differentially expressed genes. Viral genome of Sungri 96 vaccine virus was assembled from the PPRV infected transcriptome confirming the infection and demonstrating the feasibility of building a complete non-host genome from the blood transcriptome. Therefore, in this study we investigated the host-vaccine virus interactions by infecting the peripheral blood mononuclear cells isolated from goat with PPRV (Sungri 96 vaccine virus), to quantify the global changes in the transcriptomic signature by RNA-sequencing. Global gene expression changes under PPR vaccine virus infection are not yet well defined. This vaccine virus, isolated from goat works efficiently both in sheep and goat. Sungri 96 is a live attenuated vaccine, widely used in Northern India against PPR. Mass vaccination programs around the world resulted in the decline of PPR outbreaks. Peste des petits ruminants (PPR), is an acute transboundary viral disease of economic importance, affecting goats and sheep.
0 Comments
Leave a Reply. |